flowsim

Simulate 454 pyrosequencing

Provides clonesim, a clone simulator that simulates shotgun genomic clones, and flowsim, that takes the output from clonesim (or any other Fasta-formatted file) and generates simulated reads from them mimicing Roche's 454 pyrosequencing technology, writing output in 454's native SFF format. The flowgram generation is based on empirical distributions derived from real data (although analytic distributions are available too, if you prefer). The Darcs repository is at <http://malde.org/~ketil/biohaskell/flowsim>.

homepage ↗